How can we ensure that Total Worker Health research is appropriate and effective? In March 2017, the Healthier Workforce Center of the Midwest hosted a Total Worker Health (THW) Research Methodology Workshop which included national experts from NIOSH, TWH centers and affiliates. The meeting was held in response to recommendations from the Independent Panel of the Pathways to Prevention (P2P) workshop, “Total Worker Health: What’s Work Got to Do with It?” and goals provided in the National Total Worker Health Agenda. Experts reviewed current methodological approaches and limitations to TWH-related research and explored methods that have the most promise to advance the scientific evidence-base to develop a set of recommendations to guide the design of future TWH studies. Proceedings from the meeting will be submitted to the Journal of Occupational and Environmental Health.